RESEARCH PROJECTS
Development of original nanobiohybrid structure «magnetic nanoparticles-DNA» for design of biosensors
The research aimed to conjugate magnetic nanoparticles (CoFe3O4, Fe@C) and oligonucleotide / aptameres to design of magnetosensitive system for in vitro diagnostic.
Theranostic system based on superparamagnetic iron oxide nanoparticles targeted by recombinant pH-low-insertion peptide
This research aimed to engineering of the multifunctional system based on superparamagnetic iron oxide nanoparticles, which is able to provide MRI of tumor and therapeutic effect by hyperthermia. It is the two-component theranostic system in which pH-low-insertion peptide is fixed in definite orientation on APS-modified surface of the superparamagnetic nanoparticles. Superparamagnetic nanoparticles serve as MRI-contrast agent and provide therapeutic effect due to local hyperthermia production in altering magnetic field, while the pH-low-insertion peptide provides targeting to tumor in vivo. The key physicochemical and biological properties of the designed nanosystem will be investigated in vitro and in vivo and its application efficiency will be assessed.
Production of recombinant UBI 18-35 targeting peptide, labeling with radionuclide and evidence of the diagnostic efficiency in vivo in biological model
The project is focused on recombinant antimicrobial targeting peptide production and its subsequent labeling with 99mTc radionuclide including the design and synthesis of effective bifunctional chelating agent (BFCA). Radiolabeled peptide is meant for differential diagnosis of bacterial infection and inflammation which is a common complication of osteomyelitis, arthritis, as well as heart surgery, prosthetic joints and so forth. The in vivo assessment of the efficiency of differential diagnosis using radiolabeled peptide will be provided in animals’ model.
Project realization supposes the biotechnological approach for production of peptide which provided specificity and correspondingly high performance of designed system for the solving of medical diagnosis topical challenge.
